Journal: Clinical and Translational Radiation Oncology

Article Title: MonoHER selectively enhances the radiotherapy response in p53 wild-type breast cancer via stabilization of p53

doi: 10.1016/j.ctro.2026.101147

Figure Lengend Snippet: Effect of monoHER (M) combined with radiation (RT) on γ-H2AX immunofluorescence staining (at 24 h post-irradiation time point) in breast cancer and normal cells. Data are presented as mean ± SEM from three independent experiments (20 cells/experiment). *p < 0.05, **p < 0.01.

Article Snippet: Cells were incubated with a rabbit anti-human γH2AX primary antibody (1:500, Merck, JBW301), followed by detection using goat anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, 11001).

Techniques: Immunofluorescence, Staining, Irradiation

Journal: iScience

Article Title: Predicting DNA damage response using synthetic cell painting profiles and experimental analysis

doi: 10.1016/j.isci.2026.115000

Figure Lengend Snippet: K-means clustering-based stratification of DDR levels (A) Histogram and (B) boxplot show the distribution of DNA damage features representing γH2AX-positive cells in the idr-0080 dataset. In (B), data are shown as boxplots indicating the median and interquartile range with individual data points overlaid. (C) K-means clustering segmented the dataset into six clusters of varying sample sizes. Clusters 1 and 2 (black), which exhibited higher feature values, were designated as the high-DDR group in subsequent analyses. Red dashed lines indicate classification thresholds separating low and high DDR levels.

Article Snippet: Alexa Fluor®488 anti-γH2AX antibody , Cell Signaling Technology , Cat# 9719S; RRID: AB_561076.

Techniques:

Journal: iScience

Article Title: Predicting DNA damage response using synthetic cell painting profiles and experimental analysis

doi: 10.1016/j.isci.2026.115000

Figure Lengend Snippet: Application of the model to external datasets and experimental testing (A) Principal component analysis plot for the idr-0080 and cpg-0012 datasets. (B) Classification of cpg-0012 compounds into high-DDR (9,923) and low-DDR (20,694) groups. The high-DDR group includes known DNA damage inducers. Test compounds without prior DDR annotation were selected from the high-DDR (tetrindole, KF38789, and LY2183240) and low-DDR (amoxapine, acetazolamide, and captopril) groups. Flow cytometric (C) and Western blot (D) analyses of γH2AX expression in U2OS cells following 18 h treatment with 10 μM test compounds. (E) U2OS cells were treated with high-DDR candidates at increasing doses or with 10 μM low-DDR candidates for 72 h, and cell viability was measured using the CellTiter-Glo assay. Data are shown as means ± SD from independent experiments. One-way ANOVA with LSD post hoc test was used for statistical analysis. ∗ , p < 0.05; ∗∗∗ , p < 0.001 versus vehicle.

Article Snippet: Alexa Fluor®488 anti-γH2AX antibody , Cell Signaling Technology , Cat# 9719S; RRID: AB_561076.

Techniques: Western Blot, Expressing, Glo Assay

Journal: Molecular Pharmaceutics

Article Title: Attenuated Toxicity and Antitoxic Mechanism via Sodium Iodide Symporter Inhibition-Based Tumor-Selective Delivery in Astatine-211 Radioimmunotherapy

doi: 10.1021/acs.molpharmaceut.5c01438

Figure Lengend Snippet: DSB formation in the stomach and thyroid after 211 At-RIT. (A) Fluorescent immunohistostaining. The nucleus is indicated in blue, and γH2AX and NIS are indicated in red. At 3.5 h after RIT with 1 MBq of 211 At-anti-HER2 mAb (ATE), γH2AX and NIS expressions were evaluated on consecutive sections. Scale bar: 100 μm. (B) Box and whisker plots showing relative DSB accumulation in the stomach and thyroid following 211 At-RIT. Three mice were examined in each group. Number of counted cells: stomach in PBS/ 211 At-anti-HER2 mAb (ATE) group, 21,668 cells; stomach in SP/ 211 At-anti-HER2 mAb (ATE) group, 21,229 cells; thyroid in PBS/ 211 At-anti-HER2 mAb (ATE) group, 6,533 cells; thyroid in SP/ 211 At-anti-HER2 mAb (ATE) group, 4,628 cells. ***, P < 0.001.

Article Snippet: The tissue sections were blocked with 5% skim milk in PBS for 1 h at RT and then incubated with a rabbit anti-γH2AX antibody (Cell Signaling Technology, Danvers, MA, USA) diluted 1:400 in the blocking buffer for 1 h at RT.

Techniques: Whisker Assay